| 摘要 |
A thermostable amylase having the following properties: (1) Action: acting on raw starch to produce mainly maltose and matotriose; (2) Optimum pH: about 6.0; (3) Stable pH: after incubation at a pH of 5 to 8 for one hour at 22 DEG C, the residual activity thereof is at least 95%; (4) Optimum temperature: 70 DEG C; (5) Thermostability: the enzyme is not substantially inactivated by incubation at 60 DEG C or 70 DEG C for 15 minutes; and after incubation at 70 DEG C for one hour, the residual activity thereof is at least 90%; (6) Molecular weight: 52,000 +/- 5,000 as determined by gel filtration; a process for production of the amylase using Bacillus stearothermophilus; and a process for production of maltooligosaccharide comprising mainly maltose and maltotriose, comprising the steps of treating raw starch with a thermostable amylase which acts on raw starch, at a temperature lower than a temperature at which the raw starch is gelatinized to hydrolyze the starch and thereby form maltooligosaccharides comprising mainly maltose and maltotriose; and recovering the maltooligosaccharides. |
