METHOD FOR DETECTING TARGET NUCLEIC ACID IN SPECIMEN.

摘要

A method for detecting a target nucleic acid in a specimen, which comprises hybridizing in a specimen a primer of at least one strand of the nucleic acid chains of a target nucleic acid and adding a unit nucleic acid thereto for elongation of the chain to thereby prepare a synthetic nucleic acid complementary to and hybridized with said strand, thus obtaining a copy of the target nucleic acid comprising (a) the double-stranded nucleic acid thus formed, (b) a synthetic nucleic acid prepared by separating the double-stranded nucleic acid into a single-stranded nucleic acid, or (c) a double-stranded nucleic acid which is a double-stranded hybridized product complementary to the synthetic nucleic acid, and examining whether this copy exists or not in the sample.