A METHOD FOR IMMOBILIZING ENZYMES, AND IN PARTICULAR, TO A METHOD FOR CONVERTING CELL BOUND ENZYMES INTO CELL MASS PARTICLES

摘要

An improvement directed to the generation of particle form cell mass immobilized enzymes through cross-linking with polyazetidine prepolymer which comprises pretreating the enzymatically active microorganism cells in aqueous suspension with glutaraldehyde to effect partial cross-linking followed by dewatering and then admixing so-treated cell mass with the polyazetidine prepolymer followed by curing. …The dispersion of treated enzymatically active cell mass with the polyazetidine prepolymer may be sub-divided and shaped into granular form prior to curing, so that the immobilized enzyme product comprises cell mass enzyme granules of desired size and a controlled shape in high yield. …Glucose isomerase from a strain of the Streptomyces murinus cluster and tryptophan synthetase from a strain of E. coli are exemplified preferred embodiments.