| 摘要 |
<p>A new process has been developed which facilitates separation of DNA strands following the polymerase chain reaction. The process comprises 1) reacting a double stranded DNA with a biotinylated primer and a non-biotinylated primer to provide a double-stranded DNA wherein one strand has biotin bound to the extension primer; 2) reacting the product of step 1 with a support that will bind the biotin moiety; 3) denaturing the DNA; and 4) separating the amplified single-stranded DNA lacking the biotin moiety from the mixture containing the amplified DNA having biotin bound thereto.</p> |
