METHOD OF SELECTING FOR RECOMBINANT POX VIRUSES

摘要

Method of selecting for pox viral recombinants without the use of drugs, mutagens or chromogenic indicators. A pox virus which is incapable of replicating on a selective host cell because it lacks the function of a gene necessary for replication on the cell is recombined with a vector containing the gene which restores the ability of the virus to replicate on the selective host cell. A foreign gene inserted into the virus by the vector will also be expressed by the recombinant virus. The recombinant can be selected for by plating on to cells which are selective for the recombinant virus.