| 摘要 |
PURPOSE:To detect a sitotoxism bacterium, the genus Vibrio bacterium, in a short time and in a high accuracy by culturing a constant amount of a specimen in a nitritive medium and bringing the multiplied bacterium bodies into contact with benzoylargininemethylcoumalic acid in the presence of EDTA. CONSTITUTION:A constant amount of a specimen is added to a nutritive medium and cultured and the cultured liquid is centrifuged. The resultant supernatant is removed from the centrifuged liquid to separate the bodies of the multiplied Vibrio parahaemolyticus bacteria, to which is added a 7.5pH 50mM phosphoric acid buffer solution containing ethylenediaminetetraacetic acid(EDTA) and benzoylargininemethylcoumalic acid to cause a reaction therebetween. The reaction product is centrifuged to separate the supernatant, which as a specimen is measured by a spectrophotofluorimetry to determine the fluorescence strength of the specimen, thereby rapidly and highly accurately detecting the genus Vibrio bacteria. It is preferable that the nutritive medium for culturing the specimen contains common salt and polymyxin B. |
