| 摘要 |
PCT No. PCT/SE89/00241 Sec. 371 Date Jan. 5, 1990 Sec. 102(e) Date Jan. 5, 1990 PCT Filed Apr. 28, 1989 PCT Pub. No. WO89/10975 PCT Pub. Date Nov. 16, 1989.The method for demonstrating the presence of an activity of an enzyme by (a) incubating said enzyme with a fluorogenic substrate A which is converted by the enzyme to a product B differing from A in respect to its fluorescent properties, A and/or B carrying a chromophore which is a triplet sensitizer having a triplet energy level above the excitation energy level of a lanthanide ion selected from the group consisting of Eu3+, Tb3+, Dy3+ and Sm3+ and which is capable of chelating said lanthanide ion by means of an oxygen or nitrogen atom in said chromophore, and that B differs from A either by (i) carrying a different chromophore, or (ii) having a different chelating ability, and (b) measuring the change in fluorescence caused by said enzyme. |
