| 摘要 |
<p>The present invention, utilizing recombinant DNA technology, provides a novel method for obtaining 20-deoxotylosin from a tylosin producing microorganism. 20-deoxotylosin is useful as an antibiotic with a microbial inhibitory activity similar to tylosin. The method comprises culturing a tylosin-producing strain of Streptomyces, said strain: (a) comprising one or more mutations that prevent the expression of tylosin biosynthetic genes tylI and R, in which R is one or more tylosin biosynthetic genes selected from tylA, tylB, tylL, tylM, tylH, tylD, tylJ, tylG, tylK, tylC, tylE, or tylF; and, (b) being transformed with a recombinant DNA cloning vector which comprises DNA which complements said mutation or mutatons in R thereby allowing for the expression of R; said culture conditions suitable for cell growth, expression of R and production of 20-deoxotylosin.</p> |
