Method of culturing cells using highly gas saturated media.

摘要

A method of culturing cells by providing a highly gassed media on a continous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.