METHOD FOR THE PRODUCTION OF BEAD DEXTRAN MATERIALS FOR GEL CHROMATOGRAPHY

摘要

A method for the production of bead dextran materials for gel chromatography, wherein dextran is, before or during the suspension process or in both stages, subjected to reduction in an alkaline solution, advantageously by the action of sodium borohydride. The density of crosslinking is controlled above all by variation of the concentration of the initial dextran solution and the resulting gel is optionally subjected to the additional crosslinking in a separate subsequent operation. The procedure according to the invention provides the product of higher quality with a lower content of carboxylic groups and substantially facilitates the preparation of gels with a very high and a very low crosslink density.