发明名称 |
Method for producing the Nde I restriction endonuclease and methylase. |
摘要 |
<p>The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Nde I restriction endonuclease, and 3) purifying the Nde I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Nde I restriction endonuclease activity.</p> |
申请公布号 |
EP0343009(A2) |
申请公布日期 |
1989.11.23 |
申请号 |
EP19890305109 |
申请日期 |
1989.05.19 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
BENNER, JACK STANLEY |
分类号 |
C12N9/16;C12N1/21;C12N9/10;C12N9/22;C12N15/09;C12N15/54;C12N15/55;C12R1/19;C12R1/36 |
主分类号 |
C12N9/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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