METHODS OF PURIFICATION AND REACTIVATION OF PRECIPITATED HETEROLOGOUS PROTEINS

摘要

A heterologous expression product in host cells is separated from the host cell material by suspending the material in a buffered solution, disrupting the cells and separating the product as refractile material by low speed centrifugation. The refractile material can be dissolved in a strongly denaturing solution, which is then weakened while keeping the protein in solution, thereby allowing unfolding and refolding of the protein. Disulfide linkages in the protein may be cleaved, and reformed later after the protein has refolded.