发明名称 |
FREMGANGSMAADE TIL AT SKELNE MELLEM NUCLEINSYRESEGMENTER PAA BASIS AF NUCLEOTIDFORSKELLE |
摘要 |
A process for distinguishing nucleic acid segments on the basis of nucleotide differences, thereby providing a rapid, convenient means for detecting mutation, is disclosed. The present process comprises synthesizing separately complementary nucleic acid strands on each of at least two target nucleic acid templates using a nucleic acid polymerase and nucleoside triphosphate substrates, wherein at least one of the natural nucleoside triphosphate substrates is replaced with a mobility-shifting analog; denaturing the synthesized strands from the templates if necessary; and comparing the mobility of the separately synthesized strands through a size-fractionation medium. A difference in mobility indicates that the target nucleic acid templates contain different numbers of total nucleotides and/or different numbers of nucleotides complementary to the mobility-shifting analogs. Thus, the process of the present invention can be used to distinguish homologous DNAs or RNAs differing by nucleotide substitutions that affect the number of mobility-shifting analog residues incorporated into synthesized strands, as well as to distinguish differences due to nucleotide insertion or deletion. |
申请公布号 |
DK569989(D0) |
申请公布日期 |
1989.11.14 |
申请号 |
DK19890005699 |
申请日期 |
1989.11.14 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
KORNHER, JOHN STEPHEN;LIVAK, KENNETH JAMES |
分类号 |
G01N33/58;C07H21/04;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/58 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|