PROCESS FOR THE PRODUCTION OF CHOLESTEROL ESTERASE

摘要

The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: in which R and R1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R1 can also be a hydrogen atom and R2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula -CH2-(CHOH)n-CH2OH, in which n is a whole number of from 1 to 4.