| 摘要 |
This invention relates to processes for purifying acid stable phospholipase A2. Specifically, the invention relates to processes for the purification of phospholipase A2 form biological sources, such as non-pancreatic human sources which contain very small amounts of that enzyme. More specifically, this invention relates to the purification and characterization of phospholipase A2 from human platelets and from human rheumatoid synovial fluid. This invention also relates to polypeptides corresponding to at least a portion of the amino terminal amino acid sequence of human platelet and rheumatoid synovial fluid phospholipase A2 and antibodies thereto, as well as antibodies to purified, intact acid-stable phospholipase A2 for use in the treatment or diagnosis of inflammation and tissue injury associated with various diseases. And this invention relates to DNA sequences which encode these polypeptides. This invention further relates to methods for producing phospholipase A2 in hosts transformed with recombinant DNA molecules comprising those DNA sequences. |
