| 摘要 |
NEW MATERIAL:A differentiation-inducing factor existing in a culture supernatant of human lung-derived fibroblast. Molecular weight: 25000+ or -2000 (under a reduced form, SDS-PAGE method) ; isoelectric point: IU 5.4+ or -0.2; N-Terminal amino acid sequence: the formula (X is unidentified) ; Bioactivity; Showing a differentiation-inducing activity for a human tissue bulboid lymphoma-derived monoblast established cell U-937 and a human acute monocytic leukemia established cell THP-1; Specific activity: 5X10<6>-10X10<6> unit/mg.protein. USE:A chemical or pharmacological reagent and a medicine. PREPARATION:A human lung-derived fibroblast (preferably an established cell WI-26VA4, etc., obtained by phenotypic transformation of a human lung origin-derived fibroblast) is initially cultured using RPMI-1640 culture medium, etc., preferably at 37 deg.C under a saturated water vapor containing 5% CO2 concentration. The above mentioned supernatant is subsequently subjected to silica gel treatment, hydroxyapatite treatment, gel filtration, isoelectric chromatography and reversed phase chromatography in order to recover the objective substance. |
