发明名称 |
Immunoassays for antibodies which bind to the acquired immunodeficiency virus |
摘要 |
A process for the detection of antibodies to HTLV III/LAV comprising: (A) mixing an unknown serum sample with a crude HTLV III/LAV viral antigen selected from the group consisting of (1) an antigen comprising P24 core protein and Penv protein; (2) a P24 antigen and (3) a Penv antigen, (B) incubating the resultant mixture from step (A); (C) contacting the mixture of step (B) with a solid substrate coated with antibody to HTLV III/LAV; (D) incubating the mass from step (C); (E) washing the mass from step (D); (F) contacting the mass from step (E) with a labeled antibody to HTLV III/LAV; (G) incubating the mass from step (F); (H) washing the mass from step (G); (I) assaying the label in the mass from step (H); (J) as a negative control, mixing a serum sample known to be negative to HTLV III/LAV antibody with a diluent; (K) subjecting the mass from step (J) to steps (B) to (I); (L) as a positive control, mixing a predetermined amount of the crude HTLV III/LAV viral antigen and the diluent; (M) subjecting the mass from step (L) to steps (B) to (I); and (N) comparing the results of the assay for the label from steps (I), (K) and (M).
|
申请公布号 |
US4877725(A) |
申请公布日期 |
1989.10.31 |
申请号 |
US19850718140 |
申请日期 |
1985.04.01 |
申请人 |
NEW YORK BLOOD CENTER, INC. |
发明人 |
NEURATH, ALEXANDER R.;STRICK, NATHAN |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|