PRODUCTION OF THERMOSTABLE PROTEASE

摘要

PURPOSE:To eliminate dispersion in productivity under the same culture conditions and efficiently obtain the subject enzyme by cultivating nonthermophilic bacteria containing a gene capable of coding a thermophilic bacteria-delived thermostable protease. CONSTITUTION:A gene capable of coding a thermophilic bacteria-derived thermostable protease [preferably thermostable protease gene of Bacillus.stearothermophilus TELNE (FERM-P 9439)] is initially integrated into a chromosomic DNA of nonthermophilic bacteria (preferably Bacillus.subtilis and the above-mentioned bacteria are cultivated to collect the aimed enzyme from the afore-mentioned culture.