| 摘要 |
<p>Cell electrofusion apparatus, for example for the production of hybridomas, comprising a flow-through capillary (14) surrounded by a water-cooled jacket (16) and through which a physiologically normal medium (such as a tissue culture fluid) containing cells is pulsed by a pump driven syringe (28), high voltage pulses being applied to electrodes (18) at opposite ends of the channel, which voltage pulses are of a magnitude and duration such that, taking into account the rate of fluid flow through the capillary and the extraction of heat by the cooling means, the temperature of the cell-containing medium is constrained to remain below the limit at which substantial cell damage occurs.</p> |
