| 摘要 |
<p>The invention relates to a method of preparation of water-soluble macromolecular conjugates of haemoglobin, wherein:
- in a first step, sites Z are bound to a polymer P, the sites Z containing on the one hand at least one negative charge carried, for example, by sulphate groups and intended for creating an ionic bond with haemoglobin, and containing on the other hand at least ..... for example, by a carboxyl group and intended for creating a covalent bond with haemoglobin,
- then, in a second step, the polymer P containing the site or sites Z is reacted with haemoglobin in oxygenated form, to form, on the one hand at least one ionic bond between at least one of the sites Z carried by the polymer and the haemoglobin, and on the other hand at least one covalent bond between the abovementioned same site Z carried by the polymer and the haemoglobin.
<??>The macromolecular conjugates obtained by this method possess a lower affinity for oxygen than that of free haemoglobin. </p> |
