| 摘要 |
<p>The invention provides methods for amplification of a nucleic acid segment of interest wherein a DNA ligase catalyzes ligation of DNA segments hybridized to the segment of interest or the segment with the sequence complementary to that of the segment of interest. The methods of the invention have broad application to molecular biology and practical applications thereof, in which a particular nucleic acid segment needs to be detected or isolated. Among the applications of the methods of the invention is amplifying nucleic acid analytes for permitting or enhancing their detectability in nucleic acid probe hybridization assays.</p> |
