| 摘要 |
A method for the in vitro culture of the early bovine zygote to the late morula or blastocyst stage comprises incubating intact Cumulus-Oocyte-Complex under non-static conditions in a medium containing greater than 10% by weight of heat-inactivated oestrous cow serum and greater than 4 x 10<6> additional follicle cells until the oocytes reach maturation, fertilising the oocytes with capacitated bull sperm and co-culturing the zygotes thereby obtained on a monolayer of cumulus cells for 6-9 days. The zygotes are then evaluated and are either used for fresh transfers to a recipient cow or heifer or, alternatively, are stored frozen in liquid nitrogen for subsequent use. |
