A novel system of inducible positive regulation of use for production of heterologous proteins.

摘要

The description relates to a novel system of inducible positive regulation of use in the construction of vectors reproducible by expression in host microorganisms for controlled production of heterologous proteins in high yields. More particularly the system comprises the vir EcoRI-XhoI region of about 4.5 kb contained in the chromosomal DNA of Bordetella which codes for one or more regulating factors, and a terminal 5 min region situated in front of the promotor of the chromosomal Bordetella genes capable of being activated or repressed in trans by the aforementioned factors. The description also relates to vectors which can be replicated by expression and contain the regulating system and microorganisms transformed by the vectors.