| 摘要 |
PURPOSE:To obtain a large amount of dihydrofolic acid reductase-angiotensin fused protein, by manifesting a fused gene in which an angiotensin I gene is included in a recombined plasmid pLEK1 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pANG1-23, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4222 basic pairs and having a replaced structure with a conformation of 28 basic pairs containing a conformation coding leucine enkephaline between BamHI site and MluI site in pLEK1 to a chemically synthesized DNA of 43 basic pairs containing a conformation coding angiotensin I, is produced. A colon bacillus introduced of pANG1-23 produces a large amount of dihydrofolic acid reductase- angiotensin fused protein. |
