发明名称 |
ASSAY FOR HUMAN IMMUNODEFICIENCY VIRUS |
摘要 |
A method is provided for assaying for human immunodeficiency virus which comprises: seeding dishes with cells susceptible to HIV infection; adding to the dishes a sample to be tested for HIV infection; incubating the cells; exposing the cells to a first antibody reactive with HIV proteins; exposing the cells to marker-conjugated antibody reactive with the first antibody; examining the dishes with a suitable microscope; and counting the number of foci of marker-positive cells per dish to determine the number of infected cells in the dish. The process can be used to detect and to quantify HIV-infected cells, which permits monitoring of the effects of drug therapy on the levels of HIV expression in AIDS patients, as well as providing a quantitative measure of HIV expression in asymptomatic HIV antibody-positive individuals. |
申请公布号 |
AU3299689(A) |
申请公布日期 |
1989.10.05 |
申请号 |
AU19890032996 |
申请日期 |
1989.03.15 |
申请人 |
UNITED STATES OF AMERICA, REPRESENTED BY THE SECRETARY, UNITED |
发明人 |
BRUCE CHESEBRO;KATHY WEHRLY |
分类号 |
G01N33/569;C07K14/16;G01N33/577 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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