| 摘要 |
An antibiotic F-0769 having the following properties: (1) Outer appearance: White or light yellow powder, (2) Melting point: 245 DEG -250 DEG C., (3) Specific rotation: [ alpha ]D25=-37.5 DEG (c=1, methanol), (4) Solubility in solvents: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate and benzene; and insoluble in water and hexane, (5) Elementary analysis (found %): C:57.53, H:7.36, O:21.17, N:12.97, (6) Ultraviolet absorption spectrum (as measured in methanol: As shown in FIG. 1, lambda max(E1 cm1%)=213 nm (466), 286 nm (200) (7) Infrared absorption spectrum (as measured by KBr method): As shown in FIG. 2, (8) Nuclear magnetic resonance spectra (as measured in heavy chloroform): 1H-NMR spectrum is as shown in FIG. 3, and 13C-NMR spectrum is as shown in FIG. 4, (9) Distinction among basicity, acidity and neutrality: Neutral substance, (10) Amino acid analysis: As a result of the hydrolysis with 6N hydrochloric acid at 110 DEG C. for 18 hours, amino acids i.e. threonine, valine and leucine were detected, (11) Color reactions: Positive in the iodine reaction and in the potassium permanganate reaction; and negative in the ninhydrin reaction and in the ferric chloride reaction, (12) Thin layer chromatography (by means of silica gel, Art, 5715, manufactured by Merck Co.): -Solvent system Rf value -Ethyl acetate 0.16 -Ethyl acetate-methanol (5:1) 0.33 -Chloroform-methanol (10:1) 0.68 -Ethyl acetate-acetone (1:1) 0.31 -Acetone-benzene (5:1) 0.40 - (13) Molecular weight (as measured by Rast method): About 745 |
