PROCESS FOR THE PREPARATION OF L-ALPHA-AMINO ACID AND D-ALPHA-AMINO ACID AMIDE

摘要

The invention relates to a process for preparation of L- alpha -amino acid and D- alpha -amino acid amide from DL- alpha -amino-acid amide by contacting the DL- alpha -amino acid amide in an aqueous solution with an alpha -amino acid amidase containing preparation obtained from a culture of Pseudomonas putida in the presence of traces of bivalent metal ions as activator, characterized in that the aqueous solution also contains a potassium salt selected from the group consisting of potassium sulphate and potassium chloride. The invention further relates to a process for the preparation of L- alpha -amino acid and D- alpha - amino acid amide starting from the corresponding aldehyde, potassium cyanide and ammoniumsulphate, subsequent treatment with a ketone and potassiumhydroxide and finally subjecting to enzymatic hydrolysis with a preparation obtained from Pseudomonas putida.