发明名称 |
Preparation of pure human tumor necrosis factor and hybridomas producing monoclonal antibodies to human tumor necrosis factor |
摘要 |
Human TNF(LuKII) having a specific activity of at least 1.5x105 units per milligram of total protein is produced by contacting a TNF-containing protein composition, which has been harvested from human cell lines of hematopoietic origin or recombinant origin, in separate adsorption stages with glass beads, lentil lectin bound to Sepharose, and procion red agarose, thereby selectively to adsorb TNF in each stage, while leaving some impurities unadsorbed, each contact stage being followed by contact of the adsorbent with an eluant thereby to obtain a solution of more highly purified TNF after each stage. The purified human TNF(LuKII) is used to produce monoclonal antibodies against TNF(LuKII) and such antibodies can be used to assay samples for the presence of TNF(LuKII).
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申请公布号 |
US4870163(A) |
申请公布日期 |
1989.09.26 |
申请号 |
US19850770808 |
申请日期 |
1985.08.29 |
申请人 |
NEW YORK BLOOD CENTER, INC.;SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH |
发明人 |
RUBIN, BERISH Y.;ANDERSON, SYLVIA L.;SULLIVAN, SUSAN A.;OLD, LLOYD J.;WILLIAMSON, BARBARA D.;RICHARDS, ELIZABETH C. |
分类号 |
G01N33/53;A61K38/00;A61K38/16;C07K1/16;C07K1/22;C07K14/00;C07K14/005;C07K14/195;C07K14/52;C07K14/525;C07K16/00;C07K16/24;C12N15/02;C12P21/08;C12R1/91;G01N33/577 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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