METHOD FOR TRANSFORMING A CEPHALOSPORIUM CELL

摘要

A method for transforming Cephalosporium and other lower eukaryotes is disclosed. The method involves inserting a recombinant DNA cloning vector comprising a Saccharomyces cerevisiae transcriptional and translational activating sequence positioned for expression of hygromycin phosphotransferase into a host cell and then growing the host cell under selective conditions. The vectors optionally further comprise a Cephalosporium-functional autonomous replication sequence, Cephalosporium ribosomal DNA, and also sequences that allow for replication and selection in E. coli and Streptomyces.