| 摘要 |
PURPOSE:To accurately measure high-concn. pyruvic acid by using the enzyme sensor formed by using lactic dehydrogenase as immobilized enzyme and using a pH electrode or ion-sensitive FET as a transducer. CONSTITUTION:The enzyme sensor is formed by the immobilized enzyme which acts specifically on a substrate and the transducer which converts a change in the concn. or quantity of heat of a material to be consumed or formed by an enzyme reaction to an electric signal. The lactic dehydrogenase is used for the enzyme and the pH electrode or ion-sensitive field effect transistor ISFET is used for the transducer. A change in pH is measured by using a measuring instrument consisting of the ISFET 1 which is immobilized with the lactic dehydrogenase, the ISFET 2 which is not immobilized therewith and an Ag/AtCl electrode 3 in order to measure the pyruvic acid by using the enzyme sensor. The high-concn. pyruvic acid is thereby measured with the good accuracy without using a costly reagent. |
