发明名称 |
High yield extn. of high mol.wt. DNA from fish roe - by incubating in salt soln. with protease, addn. of phenol, phase sepn. then pptn. with alcohol |
摘要 |
Extn. of DNA, esp. from animal organs such as fish soft roe comprises (1) grinding the roe and suspending in a salt soln.; (2) treating with proteolytic enzymes in presence of ionic detergent; (3) treatment in suspension, with phenol to complete lysis of proteins; (4) physical sepn. of the phenol phase (contg. protein decomposition prods.) to recover a DNA-contg. liq. and (5) pptn. of DNA from this with EtOH. Specifically the NaCl content of the salt soln. is not over 1M and the enzyme used is papain. USE/ADVANTAGE - This method is rapid (only 1/6 of the time of conventional processes) and provides good yields of very pure high mol.wt. DNA on a large scale. Successive treatment with enzyme and phenol in a single reactor simplifies the process and produces a synergistic action. The prods. are useful e.g. in cosmetics for regeneration of the skin.
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申请公布号 |
FR2628440(A1) |
申请公布日期 |
1989.09.15 |
申请号 |
FR19880003279 |
申请日期 |
1988.03.14 |
申请人 |
DAVIGEL SA |
发明人 |
JEAN MOREL;ROGER GROULT;PIERRE CERUTTI;JEAN-YVES GAGNEPAIN |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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