| 摘要 |
PCT No. PCT/GB84/00189 Sec. 371 Date Jan. 30, 1985 Sec. 102(e) Date Jan. 30, 1985 PCT Filed May 31, 1984 PCT Pub. No. WO84/04757 PCT Pub. Date Dec. 6, 1984.An upstream activator sequence derived from the yeast PGK gene. The upstream activator sequence is contained in the 5' region of the yeast PGK gene between nucleotides -324 and -455. The upstream activator sequence has synthetic linkers attached to either end to facilitate attachment to a vector. Vectors including the upstream activator sequence and a heterologous promoter sequence are described. The upstream activator sequence is used in a method for increasing the expression level of a yeast expression vector. |
