Method of detecting nucleotide sequences.

摘要

A method for detecting the presence or absence of one or more variant nucleotide sequences. The method comprises (i) contacting a nucleic acid sample with a diagnostic primer which is substantially complementary to a diagnostic portion of a target base sequence, whereby extension of the diagnostic portion on a target template under appropriate conditions is only achieved where a terminal nucleotide of the diagnostic primer is complementary to either a suspected variant nucleotide or a corresponding normal nucleotide of the target base sequence, and (ii) detecting the presence or absence of an extension product. Kits for performing diagnostic tests are also disclosed.