A METHOD FOR THE AMPLIFICATION OF NUCLEOTIDE SEQUENCES

摘要

A method for the amplification of a nucleic acid fragment containing a partially unknown sequence and kits therefor are provided which enable long nucleotide sequences to be rapidly and efficiently sequenced. The method comprises the formation of target nucleic acid fragment/vectorette units by cleavage of target nucleic acid followed by ligation. One of the nucleic acid fragments will contain an initiating priming region of known sequence for hybridisation with an initiating primer and target nucleic acid fragment/vectorette units will contain a vectorette priming region of known sequence for hybridisation with a vectorette primer. Amplification is effected by primer extension of an initiating primer hybridised to the initiating priming region of the target nucleic acid fragment/vectorette unit.