Assay for ligands by separating bound and free tracer from sample

摘要

In an assay wherein there is produced in an assay sample tracer bound to a soluble binder and unbound tracer, both bound and unbound tracer are separated from the assay sample for separate determination thereof by use of a supported binder for the soluble binder and a supported binder for the tracer. The assay may be effected as a "flow-through assay" by causing the sample to flow through successive chambers containing the supported binders.