POLYPEPTIDE POSSESSING CYCLOMALTODEXTRIN GLUCANOTRANSFERASE ACTIVITY

摘要

The sequence of cyclomaltodextrin glucanotransferase (CGTase) gene derived from a microorganism of the genus Bacillus and the amino acid sequence of CGTase are determined. A recombinant DNA carrying the CGTase gene is introduced by an in vitro genetic engineering technique into a host microorganism of species Bacillus subtilis or Escherichia coli. The recombinant microorganism carrying the recombinant DNA automically proliferates to secrete a large amount of CGTase. The enzymes are used to produce glycosides having low calorie low cariogenic properties.