摘要 |
The present invention provides an apparatus and method for fluorescence detection. An ellipsoidal cylinder having a first focal line, a second focal line and an internal reflective surface is provided. An excitation source is disposed on the first focal line and a sample container is disposed on the second focal line. Filter means are disposed between the excitation source and the sample container. Both fluorescence detector and reference detector means are provided to detect, respectively, the fluorescence radiation and excitation radiation. Comparator means compare the signal from the fluorescence and reference detectors to determine the presence and concentration of fluorescent material within the sample container.
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