| 摘要 |
Flash fluorimetric analysis of a specimen expected to produce a first, specific, fluorescent radiation on excitation at a test position, including in the performance of a ''blank'' analysis: supporting at said test position a reference fluorophore expected to produce a second, characteristic, reference radiation on excitation, applying to said test position a pulse of excitation radiation, examining the radiation from the test position with a respective detector for the first radiation and a respective detector for the second radiation, determining from the relative response of the detectors a ''blank'' value for the ratio of radiation intensities from the test position in the absence of a specimen, and in the similar performance of a specimen analysis: supporting at said test position in the maintained presence of the reference fluorophore a specimen expected to produce said first specific radiation on excitation by incident radiation, examining the radiation from the test position with said respective detectors, determining from the relative response of the detectors a specimen value for the ratio of radiation intensities from the test position in the presence of a specimen, and assessing said ''blank'' and specimen values to analyse the specimen with regard to a calibration step. |
