摘要 |
<p>Capillary electrophoresis apparatus includes an electronically controlled valve system 97 for automatically introducing a sample 93 into the capillary 13 by means of a vacuum at the end of the capillary tube. Such sucking in of the sample is extremely accurate and reproducible, results in a minimum of band broadening and enables the entire system to be easily automated. Automated temperature control enables the temperature of the capillary tube (and hence the solvent/solute system) to be controlled during electrophoresis, thereby controlling pH and electrophoretic mobility. The capillary may be prewashed and equilibrated to achieve substantially zero charge on the capillary wall, thereby essentially eliminating electroosmotic flow and substantially improving resolution.</p> |