| 摘要 |
PURPOSE:To obtain plasmid pTP12 having about 1.7Md mol.wt., by separating from Corynebacterium xerosis, transforming Bacillus subtilis with it, reseparating plamid, scissoring the plasmid with a restriction enzyme, ligating it. CONSTITUTION:Plasmid pTP10 is separated from Corynebacterium xerosis, Bacillus subtilis is transformed with it, and plasmid pTP11 is reseparated. Plasmid pTP11 is scissored with restriction enzyme Xba I and ligated. Consequently, plasmid pTP12 having about 1.7Md mol.wt. and cleavage map of restriction enzyme shown by the fig. is obtained. Corynebacterium xerosis strain has resistant gene to erythromycin, kanamycin, etc. on plasmid R, and plasmid pTP12 is expected to have effective use. |
