| 摘要 |
The hemostatic agent of the present invention is obtained by the following process: An aqueous solution of Trimeresurus okinavensis venom is treated with almost the same volume of an organic solvent such as chloroform, ether and ethyl acetate to remove lipids, and the water layer is collected and dialysed against a distilled water. Dyalysate is passed through dextran gel column, said dextran gel having a molecular weight fractionation range of 1,000 to 50,000, to adsorb thereon and eluted with phosphate buffered solution (pH 7 - 8). The fraction having strong coagulating activity is collected in the recalcification test and passed through a column filled with a basic ion exchange resin to allow the active substance to adsorb thereon and eluted with ethyl acetate (pH about 7). The fraction having the highest blood coagulating activity is collected in the recalcification test, and lyophilised. |
