ADDITIVES FOR USE IN CELL CULTURE CULTURE MEDIUM FOR NON - SERUM CULTIVATION AND METHOD OF CELL CULTURE USING THEM

摘要

Additives for cell culture were prepd. Thus, proteins obtained from 20 eggs were soaked in 25mM 3HCl buffer (pH 7.6) contg. 1% NaCl and treated with PEG 7500 during centrifugation (10,000 rpm X 10 min). The resulting precipitate was passed through sepharose CL-6B to give an additive (1 M.W.=700,000)