| 摘要 |
<p>PURPOSE:To obtain anti.HD monoclonal antibody from human spleen lymphocytes and myeloma cells by culturing human spleen lymphocytes in a specific serum-free medium and priming them with HD antigen. CONSTITUTION:Human spleen lymphocytes are cultured in a serum-free medium containing both antihuman globulin mu-chain antibody and supernatant of the culture mixture of human lymphocytes stimulated with concanavalin A and primed with HD antigen. The subject anti.HD monoclonal antibody is prepared from the hybridoma between human spleen lymphocytes and myeloma cells. As an HD antigen, can be used HD3, HD5, HD7 or their chemical derivatives, but HD3 is preferred. As an antihumanglobulin mu-chain antibody, is used the sheep antihuman 1gmu-chain fraction (ab').</p> |
