| 摘要 |
PCT No. PCT/DE83/00003 Sec. 371 Date Sep. 14, 1983 Sec. 102(e) Date Sep. 14, 1983 PCT Filed Jan. 13, 1983 PCT Pub. No. WO83/02404 PCT Pub. Date Jul. 21, 1983.A method and apparatus for the separation of cells, organelles or proteins from a suspension or solution thereof, including: (a) coating ferromagnetic beads with an adsorption layer comprising antibodies, haptenes or chemical substrate adapted for binding the cells, organelles or proteins to be separated; (b) introducing said beads to a separation chamber having an entrance port and an exit port, the ratio of total volume of said beads to the chamber volume being of between 0.01 and 0.5, said chamber having magnetic field gradients in the regions of said entrance and exit ports to prevent the beads from leaving the chamber through the entrance or exit ports; and (c) passing the suspension or solution containing said cells, organelles or proteins into the entrance port through the chamber and out of the exit port to effect adsorption of the cells, organelles or proteins on said adsorption layer of the beads.
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