Method for separating nucleic acid.

摘要

A solution of a mixture containing a nucleic acid to be separated is passed through a column packed with granular hydroxyapatite to retain the nucleic acid on the column, the nucleic acid then being selectively eluted by a solution of an ammonium salt of carbonic acid, to enable the nucleic acid to be effectively separated at high purity. Since hydroxyapatite is used for the packing material, the eluted nucleic acid fraction does not contain inorganic salts except the eluent component which, being an ammonium salt of carbonic acid can be decomposed into ammonia and carbon dioxide by treating under reduced pressure and/or with warming, and thus can be easily and quickly removed from the fraction.