发明名称 |
Method for determining thioredoxin reductase activity |
摘要 |
The present invention provides a method for measuring the activity of thioredoxin reductase in mammalian cells by contacting the cells in vivo or in vitro with a quaternary ammonium salt spin-labelled with a stable nitroxide free radical, and measuring the rate of reduction of the uncomplexed nitroxide free radical at the cell surface by electron spin resonance. This rate of reduction provides a measure of thioredoxin reductase activity at the surface of the cells.
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申请公布号 |
US4849346(A) |
申请公布日期 |
1989.07.18 |
申请号 |
US19870013671 |
申请日期 |
1987.02.12 |
申请人 |
REGENTS OF THE UNIVERSITY OF MINNESOTA |
发明人 |
WOOD, JOHN M.;SCHALLREUTER, KARIN U. |
分类号 |
C12Q1/26;G01N24/10 |
主分类号 |
C12Q1/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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