| 摘要 |
PURPOSE:To make it possible to obtain a bacterium of the genus Rhodococcus having extremely powerful nitrile hydratase activity, by cultivating a bacterium of the genus Rhodococcus having the ability to produce nitrile hydratase in a culture medium containing an organic acid added thereto. CONSTITUTION:A bacterium, belonging to the genus Rhodococcus and having the ability to produce nitrile hydratase is cultivated to produce bacterial cells having nitrile hydratase enzymic activity. In the process, an organic acid is added to a culture medium. At least one selected from propionic acid, n-butyric acid, isobutyric acid, n-valeric acid and isovaleric acid is preferably used as the organic acid. The concentration of the organic acid in the culture medium is normally about >=0.1-<20g/l. |
