| 摘要 |
PURPOSE:To obtain a xylooligosaccharide derivative useful in various starch industries, in high efficiency, by contacting a specific enzyme with xylan or hydrolyzed xylan in the presence of an alcohol. CONSTITUTION:A microbial strain of genus Acremonium (e.g. Acremonium cellulolyticus) capable of producing xylooligosyl transferase A is cultured in a medium containing a vegetable biomass (e.g. bagasse) as a carbon source and a nitrogen source (e.g. peptone) at 20-40 deg.C for 2-15 days under aerobic condition. The cultured product is heat-treated and purified to obtain a xylooligosyl transferase A having a molecular weight of 51,000, an isoelectric point of about 5.0, a working pH of 3-6 and an optimum working temperature of 50 deg.C. The enzyme A is added to a reaction liquid containing xylotetraose and a 1-10C straight-chain alkyl alcohol, etc., and made to react at about 45 deg.C for about 2hr to obtain the objective xylooligosaccharide derivative. |
