发明名称 |
PROCESS FOR IN VITRO PRODUCTION OF SERUM-FREE AND MITOGEN-FREE INTERLEUKIN-2 |
摘要 |
An in vitro cell culture process for producing in high yield and purity a serum-free and mitogen-free interleukin-2 containing conditioned supernatant is described. The incubation steps during stimulation and conditioning of the interleukin-2 producing cells is carried out with a rapidly rotating roller bottle culture system. Yields of interleukin-2 are on the order of 10-fold and higher than obtained by similar cultivations carried out in flat dishes or tubes or in a roller bottle culture system rotated at conventional speeds. Improvement in yields in both the static and roller culture systems are also achieved by incubating the interleukin-2 producing cells under high oxygen concentrations, for example, atmospheres of at least 70% O2 and at least 5% CO2. In addition to using peripheral mononuclear blood cells (PBL) as the source of leukocytes containing the interleukin-2 producer cells, it is also possible to use buffy coat cells which are readily available as a waste by-product from blood banks. |
申请公布号 |
DE3478310(D1) |
申请公布日期 |
1989.06.29 |
申请号 |
DE19843478310 |
申请日期 |
1984.02.10 |
申请人 |
HOOPER TRADING CO. N.V. |
发明人 |
FABRICIUS, HANS-AKE;STAHN, ROLAND |
分类号 |
C07K14/55;C12N5/02;(IPC1-7):C12P21/02 |
主分类号 |
C07K14/55 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|