Method for the quantitative determination of physiological components in biological fluids

摘要

A novel enzyme of bilirubin oxidase and a conventional enzyme of laccase are found to oxidize bilirubin to biliverdin without formation of hydrogen peroxide, thereby permitting the content of conjugated bilirubin in biological fluid to be quantitatively determined by reacting therewith the bilirubin oxidase of a microorganism of genus Myrothecium origin, and also permitting the content of total bilirubin in biological fluid to be quantitatively determined by reacting therewith such a bilirubin oxidase or laccase in the presence of a specific additive compound.